Antimicrobial Activity of Soil Isolates Essay -- Biology Microbiology

Antimicrobial Activity of Soil Isolates Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based on the amount of microbial colonies available. The Antimicrobial Activity of three different microbes were also tested and results were obtained For two out of three of the microbes. Two out of three of the tested microbes Exhibited antimicrobial activity towards the bacteria E. Coli and B. Mycoides And the results were recorded. Microbes produce various antibiotics and by Isolating different microbes the antibodies can be tested for. Introduction: This lab focuses on the isolation and characterization of an unknown organisms expressing interesting properties in relation to Antimicrobial Activity. At the conclusion of this laboratory experience The researcher would be required to describe the isolates obtained from the soil macroscopic and microscopic appearance, Perform the gram stain of the microorganisms in question and to discriminate the organisms from other microbes that could be contained in the soil and to finally make certain that the organisms have not been exposed to outside sources and be isolated in a pure culture. The researcher must first isolate the microbe and try to grow the organism in a pure culture to commence with testing the organism. The isolation of Microbes in a laboratory and clinical settings are of the up most importance and due to the isolation of different microbes from each other various procedures become open for the researcher to use like six fold dilutions that allow for the quarantined m icrobe to be reduced down in number to ensure that the test are being run are being tested on just a single type of organism. But before dilutions can occur the microbe must first be liberated from the soil and streaked onto an Agar plate to grow. There are various methods that can be used once the organism has grown onto the agar plate to ensure that the sample collected would o... ...actors due to the large or small amount of colonies present on my agar plated media. This could be accounted for by stating that there was a very large amount of microbes present in the soil and the dilutions did not dilute enough of the microorganism out or human error could also account for Not having any statistically significant plates to use. The bacterium that I replated was obtained from the YPD 10-3 media and it gave rise to bacterium 1 & 3, The other bacterium that I obtained came from LB 10-5 . The two bacterium from the YPD plate varied in color the bacterium that I refer to as one had a reddish color had a circular form a raised elevation and a entire margin, The bacterium that I refer to as 3 Had a grey appearance a circular form a raised elevation and the margin appeared to be entire. The bacterium that I obtained from the LB media had a white appearance had a circular form a faintly raised elevation and the margin was entire. References: -Microbiology Laboratory Manual -http://www.talron.co.il/index.php?module=pagemaster&page user op=view page&PAGE id=8&MMN position=9:2 -http://www.sigmaaldrich.com/img/assests/6840/95762_YPD_Agar_17119_83055_ 1777 .pdf

Gore’s movie, “An Inconvenient Truth” Essay

Former Vice President and former Tennessee Senator Al Gore discusses the correlation between carbon dioxide and other so-called greenhouse gases in the atmosphere and global warming. Gore’s movie, â€Å"An Inconvenient Truth† is constructed to look as though the majority of it is taking place in a college lecture hall, thus implying that his information is indeed fact. Better yet, before the end of the movie, Gore attempts to prove that global warming is not an environmental theory, but is a fact and that it is being caused by things that man is doing. His simple conclusion is that mankind is creating more greenhouse gases and that is causing the earth’s temperature to rise at a rate that might soon be catastrophic. Gore spends a great deal of the movie in front of a double line graph comparing carbon dioxide levels in the atmosphere and the earth’s temperature. He explains where his personal interest in the concept came from, a research professor when he was an undergraduate student, and how the professor’s work has predicted the climbing earth temperatures. Gore never specifically illustrates how mankind is contributing to the greenhouse gases, but he does make allusions to heavy traffic and coal-fired energy plants as the cause. Instead, he uses a very effective melding of classroom instruction and beautiful natural footage of his farm in Tennessee and the ice flows of Antarctica. He even compares the need to do something about global warming to his family’s failure to do something about smoking. Gore points out that until his sister died from lung cancer, his father raised tobacco on their Tennessee farm. It wasn’t until his daughter’s death that he made the connection between his action and her illness. Likewise, Gore said, he is afraid that we will not make the connection between our actions and global warming until our children are dying from it. The use of analogical reasoning is impressive through the film as is the use of pop culture to define his arguments, relying on a clip from the cartoon sit-com Futurama as his explanation of why greenhouse gases lead to heating the environment. The biggest failing of the movie are Gore’s lack of an answer for the problem he presented. He points to the ever-rising global temperature and the melting ice flows in the Arctic, Antarctica and Greenland, but only as the final credits roll are there any suggestions for what to do about it. And these claims are the ones that are least supported. For example, the credits proclaim the advantages of switching to mercury-based florescent light bulbs instead of traditional incandescent light bulbs, claiming that the energy saved by making little changes like that can help offset the growing carbon problem. But he never backs up those claims with facts and never discusses other issues which might be a factor in choosing light bulbs, such as quality of light provided and the fact that fluorescent bulbs contain mercury, a potentially lethal heavy metal. Gore’s explanations seem sound and reasonable, but that may also be due to the way they are presented. With an ice shelf breaking apart before your eyes, it is hard to demand answers about why the shelf is breaking apart. And, no one in the movie ever challenges Gore’s opinions or facts. He claims that when opponents to the global warming theory make objections that he researches them and adds the answer to his lectures, but there is no evidence of this. Instead, it appears that Professor Gore has used his training in academia and politics to make it look as though he knows all the answers, but the real â€Å"Inconvenient Truth† is that the movie leaves you with more questions than answers.

How Does Steinbeck Present the Plight of the Minorities in...

1930’s America saw many different groups within society be prejudiced against. At the time minority groups had no standing within society. Steinbeck; in his book Of Mice and Men, presents the common minority groups within society in 1930’s America and how they were forced to live their lives. The book references four characters that all represent different groups seen as minorities at the time that the book is set. This essay will follow the characters in the book and explore how and why they found themselves as minorities and how this ultimately changed the characters lives. One of the lead characters in the book, Lennie, from the start is shown as being different from others (including the other main characters George). In Of†¦show more content†¦The dog that he describes as owning from a pup almost describes him, as he is old and haggard like the dog and struggles to have any fun as he is old, still on the farm and is missing his hand. Being part of the eld erly group within society makes his dream unobtainable, but only just as he is wealthy. This is true for so many old people, as soon as they have enough money to achieve their dream they are too old to have as much fun as theyShow MoreRelatedAn Inspector Calls By. Priestley1741 Words   |  7 Pageswrote ‘An Inspector Calls’ in 1945 during the Second World War, however the play was set in 1912 just before World War 1 and the sinking of the Titanic. He did this to show the audience that society wasn’t equal. Both authors write in times that present issues of sexism, racism and social divide between the working class and the upper class. The play was first performed in 1946; Priestley became increasingly aware of social inequality of life in Britain and believed that people should take responsibility